Isolasi Enzim Bromelin dalam Bentuk Serbuk dari Buah Nanas
Pineapple fruit preferred by people, because it is have sweet to fresh sour taste and contains quite nutrients. Pineapple can be consumed as fresh fruit or be processed to some kind of food or beverage such as jam, syrup, and sticky pineapple. Besides that, pineapple usually used as meat tenderizer because it
contains bromelain enzyme, which is protease enzyme that can hydrolyze proteins. The enzyme bromelain may also be used in the production of fish soy sauce and used as a supplement. To increase effectiveness in its use, there should be a process of isolation to get the pure enzyme bromelain. The purpose of this
research is to isolate bromelain enzyme from pineapple and to compare yield and activity of the bromelain enzyme as product from organic solvent ractionation, salt precipitation, and membranes separation. The parameter used in this research include is Smooth Cayenne which had been ripe; ratio of feed and acetone 1:1; centrifugation for 8 minutes on 6000 rpm; pressure of microfiltration is 1 kg/cm2, and temperature of drying at 40oC. Whereas, the variables include part of pineapple fruit such as fruit skin and mixture of pulp with stem pineapple; separating process such as precipitation with acetone, precipitation with (NH4)2SO4, and ultrafiltration; concentration of acetone are 30%, 50%, and 70%; saturation range of (NH4)2SO4 are 0-20%, 20-40%, 40-60%, and 60-80%; and pressure of ultrafiltration are 0,6; 1; and 1,4 kg/cm2. The highest yield is 0,563%, obtained from ammonium sulfate precipitation with saturation range 0-20% on fruit skin and the highest activity of enzyme is 1820 GDU/g, obtained from membranes separation with ultrafiltration pressure is 0,6 kg/cm2 on mixture of pulp with stem pineapple. With this value of activity, the product of this research fulfills the standard quality of the enzyme bromelain which is used as a supplement.
Made by : Ronny Kurniawan, S.Juhanda, Aditia Nugraha, Irfan Djatnika Email : ron_itenas@yahoo.com
Keywords: pineapple, bromelain, isolation, precipitation, ultrafiltration.
Adverb : Prosiding Seminar Nasional Teknik Kimia “Kejuangan” Pengembangan Teknologi Kimia untuk Pengolahan Sumber Daya Alam Indonesia Yogyakarta, 5 Maret 2013 Program Studi Teknik Kimia, FTI, UPN “Veteran” Yogyakarta E3 – 1 ISSN 1693-4393